Figure 3.

Infection of P. papatasi sand flies with L. major LPG1-targeted null mutants. (A) Flies were fed on mouse blood containing 2 × 10⁶ promastigotes per ml and were dissected on day 2 (stippled bars) or day 7 (solid bars). (B) Flies were fed on mouse blood containing 10⁷ promastigotes per ml, and midguts were dissected on day 3 (stippled bars) and on day 5, at which time midguts were grouped according to those that still retained blood (light solid bars) and those that had no detectable blood (dark solid bars). Graphs show the means ± SEM of promastigotes per midgut from a single experiment (10–12 flies per group). The percentages of flies positive for parasites in each group are shown.