Figure 4.
Infection of P. argentipes sand flies with L. donovani R2D2 and C3P0 mutants. Flies were fed on mouse blood containing 2 × 106 promastigotes per ml, and midguts were dissected on day 2 (stippled bars) and day 5 (solid bars) after feeding. Graphs show the means ± SEM of promastigotes per midgut, from a pool of three separate experiments (22–30 individuals flies) for day 2 and two separate experiments (14–19 total flies) for day 5. The percentages of flies positive for parasites in each group are shown.