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Double staining of lactotransferrin (LF) mRNA in situ hybridization and D-related human leukocyte antigen (HLA-DR) immunohistochemistry in the cerebral cortex of an Alzheimer’s disease case. LF mRNA is expressed in a subpopulation of HLA-DR-positive reactive microglia (black arrows in A and B). Some LF mRNA-positive cells which are negative for HLA-DR are seen in the parenchyma of the cerebral cortex (red arrow in B) and in the vicinity of microvessels (red arrow in C). Bars = 50 μm.
