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Double staining of lactotransferrin (LF) mRNA in situ hybridization and CD4 (A), CD8 (B) or leukocyte common antigen (LCA) immunohistochemistry (C) in the cerebral cortex of an Alzheimer’s disease case. LF-positive signals (arrows in A and B) were negative for CD4 (arrowheads in A) and CD8 (arrowheads in B). Granular signals for LF mRNA are seen in the cytoplasm of LCA-positive cells (arrows in C). Bar = 50 μm.
