Figure 1.

Cross-linkable form of clathrin. (A) Design of the EGFP-FKBP-LC fusion construct (top) and the presumed integration of this construct into triskelia. (B) EGFP-FKBP-LC is preferentially expressed relative to endogenous clathrin light chain molecules in TRVb-EFL cells. Equal amounts of protein were loaded in each lane. (C) EGFP-FKBP-LC puncta (top) in TRVb-EFL cells colocalize with immunofluorescence derived from clathrin heavy chain puncta (bottom). Bar, 5 μm. (D) Distribution of the relative intensity of fluorescence derived from EGFP-FKBP-LC (LC) or immunofluorescence against clathrin heavy chain (HC) indicates that all clathrin pits incorporate the EGFPFKBP-LC fusion construct (n = 8; 800 puncta total).