Figure 5. Exotest on unfractioned samples.

(A) The amount of detectable exosomes was measured in purified exosomes (50 µg), unfractioned culture supernatants (50 µl) from human macrophages, melanoma cells and plasma from melanoma patients. Data are expressed as means±SD. (B) Regression analysis of plasma levels of CD63+ exosomes measured in purified or unfractioned plasma samples from both patients (n = 9, black diamonds) and healthy donors (n = 4, white diamonds). Exosomes levels are expressed as OD₄₅₀×1000.