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The cytoplasmic tail of CPE interacts with ARF6. In vitro binding assay of fusion proteins shows interaction of six amino acids of CPE C-terminus with active form of ARF6. (A) Input (10%) of fusion proteins was analyzed by SDS-PAGE followed by staining with Silver Stain kit. (B) Fusion proteins, bound to S-agarose beads were analyzed by Western blot. Nitrocellulose membrane was probed with anti-GST ab (top) or with Ponceau S (bottom).
