Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Mutation of the CPE cytoplasmic tail prevents its interaction with active form of ARF6. In vitro binding assay of fusion proteins shows two mutations that prevents interaction of CPE C terminus with ARF6-Q67L. Fusion proteins, bound to S-agarose beads were analyzed by Western blot. Nitrocellulose membrane was probed with anti-ARF6 ab (top) or with S-protein-HRP conjugate (bottom).
