Table 1.
Primers used to identify AFLP loci
| Primers (E-FAM) | Loci | Primers (E-HEX) | Loci | Primers (E-NED) | Loci |
|---|---|---|---|---|---|
| EGA_MGA | 48 | ECA_MCG | 40 | ECG_MCG | 12 |
| EGA_MGC | 38 | ECA_MCC | 39 | ECG_MCC | 16 |
| EAC_MCC | 47 | ECA_MGG | 56 | ECG_MTA | 38 |
| EAC_MGA | 55 | EAG_MTA | 36 | ECG_MGA | 20 |
| EAC_MTC | 89 | ECA_MCT | 59 | ECG_MTT | 32 |
| EAC_MCG | 38 | EAG_MGG | 40 | ECG_MGC | 26 |
| EAC_MGG | 67 | EAG_MCT | 47 | ECG_MGT | 21 |
| EAC_MGC | 66 | EAG_MCA | 46 | ECG_MCA | 21 |
| EAC_MGT | 43 | EAG_MTT | 46 | ECG_MGG | 26 |
| EAC_TCC | 40 | EAG_TTA | 22 | ECG_TGT | 46 |
| EAC_TAG | 61 | EAG_TCA | 39 | ECG_TAC | 25 |
| EAC_TGA | 39 | EAG_TGC | 32 | ECG_TCA | 19 |
| EAC_TTC | 60 | EAG_TAA | 32 | ECG_TGA | 47 |
Primer combinations are indicated by restriction enzyme site (E = EcoRI, M = MseI, T = TaqI) and selective dinucleotide. The Eco-primers were labelled with FAM, HEX and NED as indicated. The number of polymorphic loci identified with each primer combination is indicated.