Figure 4.

Depletion of KLP-18 by RNA interference. (A) Equal amounts of protein from wild-type and klp-18(RNAi) young adults were immunoblotted after depletion of KLP-18 via injection or feeding of dsRNA. In both cases, KLP-18 was almost undetectable. The blot was reprobed with anti-α-tubulin antibodies as a loading control. (B–D) In comparison to wild-type, klp-18(RNAi) meiotic spindles at equivalent stages showed either greatly reduced levels of KLP-18 staining (C; n = 9) or no detectable staining (D; n = 9). Anterior is to the left. Bar, 5 μm.