Table 1.
Density of VSVG within trans-Golgi/TGN and GPCs
| trans-Golgi/TGN | GPCs | |
|---|---|---|
| Nanogold | ||
| 20°C block release | 17.4 ± 4.7 | 18.4 ± 5.2 |
| Steady statea | 7.6 ± 3.1 | 8.6 ± 3.8 |
| Cryogold | ||
| 20°C block release | 11.2 ± 4.3 | 11.0 ± 3.6 |
| Steady statea | 3.8 ± 2.2 | 4.4 ± 2.8 |
Linear density of gold labeling for VSVG expressed as the number of gold particles per micrometer of membrane length. Values represent means ± SDs obtained by analyzing 25 cells (nanogold) and 18 cells (cryogold).
a
Cells were infected with VSV, kept for 4 h at 32°C, and then fixed for EM