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. 2003 Nov;14(11):4486–4498. doi: 10.1091/mbc.E03-02-0095

Figure 1.

Figure 1.

Epistatic relationships among FEAR network components. (A) mad1Δ (A2853), mad1Δ GAL-ESP1 (A7619), mad1Δ GAL-SPO12 (A6490), and mad1Δ GAL-CDC5 (A7297) cells carrying a CDC14-3HA fusion were arrested in G1 in YEP medium containing 2% raffinose (YEPR) with α-factor (5 μg/ml) for 3 h. Cells were then released into YEPR medium containing 2% galactose (YEPRG) lacking pheromone at 25°C. The percentage of cells with metaphase spindles (close triangles), anaphase/telophase spindles (close circles) as well as the percentage of cells with Cdc14-HA released from the nucleolus (open circles) was determined at the indicated times. (B) mad1Δ (A2853) mad1Δ spo12Δ bns1Δ (A5408), mad1Δ spo12Δ bns1Δ GAL-ESP1 (A7619) mad1Δ spo12Δ bns1Δ GAL-CDC5 (A7578), mad1Δ slk19Δ (A4302), mad1Δ slk19Δ GAL-ESP1 (A7302), mad1Δ slk19Δ GAL-SPO12 (A7581), mad1Δ slk19Δ GAL-CDC5 (A8032) cells carrying a CDC14-3HA fusion were grown and analyzed as described in (A). (C) mad1Δ (A2853), mad1Δ MET-CDC5 (A6560), mad1Δ MET-CDC5 GAL-ESP1 (A6562), and mad1Δ MET-CDC5 GAL-SPO12 (A6630) cells carrying a CDC14-3HA fusion were grown in medium lacking methionine and arrested in G1 with α-factor (5 μg/ml). Galactose (2%) was added 1 h before release, and cells were released into YEPRG medium containing 8 mM methionine at 25°C. The percentage of cells with metaphase spindles (closed triangles), anaphase/telophase spindles (closed circles) as well as the percentage of cells with Cdc14-HA released from the nucleolus (open circles) was determined at the indicated times.