Table 1. List of all lpxL1 mutant strains.
Strain no. | Isolated from | serogroup | ST | Clonal complex | Type of mutation in lpxL1 a |
lpxL1 mutants among 56 serogroup Y isolates | |||||
HF13 | Ndb | Y | nd | nd | V |
2011169 | blood | Y | 23 | 23 | V |
2040760 | blood | Y | 23 | 23 | V |
970455 | blood | Y | nd | nd | V |
2050913 | joint puncture | Y | 2786 | 23 | V |
971523 | CSF | Y | nd | nd | V |
971886 | CSF | Y | nd | nd | VI |
982195 | blood | Y | nd | nd | VI |
2040608 | CSF | Y | nd | nd | I |
lpxL1 mutants among 114 isolates representing major serogroups and clonal complexes | |||||
2000569 | blood | X | 750 | 750 | V |
2011833 | blood | C | 3553 | 269 | V |
2041268 | blood | B | 4926 | 35 | V |
2050093 | blood | B | 461 | 461 | V |
2030162 | blood | C | 337 | 41/44 | V |
2051372 | blood | B | 461 | 461 | V |
2010151 | blood | C | 461 | 461 | IV |
2021270 | CSF | C | 461 | 461 | III |
2041396 | CSF | B | 4930 | 18 | III |
2050806 | CSF | B | 213 | 213 | III |
2071416 | blood | Y | 23 | 23 | VI |
2020799c | CSF | B | 35 | 35 | conserved amino acid change |
2050392 | CSF | B | 213 | 213 | VII |
lpxL1 mutants among multiple isolates from a single patient | |||||
Patient 94176 | |||||
941761 I | CSF | C | nd | nd | conserved amino acid change |
941761 III | Troat swab | C | nd | nd | wild-type |
Patient 9707010 | |||||
970710 I | CSF | C | nd | nd | wild-type |
970710 III | nose swab | C | nd | nd | IV |
Patient 971859 | |||||
971859 I | CSF | C | nd | nd | IV |
971859 III | Throat swab | C | nd | nd | wild-type |
lpxL1 mutants among isolates from 254 patients in the prospective cohort study | |||||
2012202 | CSF | B | 41 | 41/44 | V |
2020434 | CSF | C | 11 | 11 | V |
991174 | CSF | C | 11 | 11 | V |
990576 | CSF | B | 571 | 41/44 | V |
991382 | CSF | B | 191 | 41/44 | V |
2011833 | CSF | C | 3553 | 269 | V |
991344 | CSF | B | 42 | 41/44 | III |
2000607 | CSF | B | 40 | 41/44 | III |
2000311 | CSF | B | 461 | 461 | III |
991093 | CSF | B | 5451 | 32 | III |
2020622 | CSF | B | 5458 | 41/44 | IV |
990344 | CSF | B | 5449 | 41/44 | IV |
2010640 | CSF | B | 1474 | 41/44 | conserved amino acid change |
2011334 | CSF | C | 11 | 11 | II |
2011764 | CSF | B | 303 | 41/44 | conserved amino acid change |
992008 | CSF | B | 146 | 41/44 | Not detected |
Type of mutations found in lpxL1. Colors in parentheses correspond to colors shown in Figure 2. Type I mutation: insertion of IS1301 (pink), type II mutation: insertion of IS1655 (pink), type III mutation: deletion of a guanine in a stretch of five guanines (yellow), type IV mutation: deletion of an adenosine in a stretch of five adenosines (green), type V mutation: deletion or insertion of an adenosine in a stretch of seven adenosines (light and dark blue), type VI mutation: deletion of ten nucleotides (red), type VII mutation: deletion of C-terminal part of the lpxL1 gene (gray). For all strains with conserved amino acid changes, the inactivation of lpxL1 has been confirmed with analysis of the lipid A by mass spectrometry.
Nd: not determined.
Strain 2020799 was part of both the panel of 114 isolates representing all major serogroups and clonal complexes and the panel of 254 isolates from patients in the prospective cohort study.