Data as mean ± SD. x axis indicates time after CLP; y axis, normalized density; filled squares, IL-6 +/+ mice; open squares, IL-6 +/+ mice administered rhIL-6; filled diamonds, IL-6 -/- mice; open diamonds, IL-6 -/- mice administered rhIL-6. Values were determined using Northern Blotting (see text). Blots were subjected to autoradiography and densitometry. Data for Ntcp, MRP-2, and OATP normalized to values for the constitutively transcribed β2M at the same time point. Values at time 0 were arbitrarily set at 100. For Ntcp, values for IL-6 +/+ and IL-6 +/+ + rhIL-6 were significantly different than T0 at 3, 6, 16, 24, and 48 h. Values for IL-6 -/- + rhIL-6 were significantly different than values for T0 at 3, 6, 16, and 24 h. Values for IL-6 +/+ and IL-6 +/+ + rhIL-6 were significantly different than values for IL-6 -/- at 3, 6, 16, 24, and 48 h. Values for IL-6 -/- + rhIL-6 were significantly different than values for IL-6 -/- at 3, 6, 16, and 24 h. For MRP-2, values for IL-6 +/+ and IL-6 +/+ + rhIL-6 were significantly different than T0 at 3, 6, 16, 24, and 48 h. Values for IL-6 -/- + rhIL-6 were significantly different than values for T0 at 3, 6, and 16 h. Values for IL-6 +/+ and IL-6 +/+ + rhIL-6 were significantly different than values for IL-6 -/- at 3, 6, 16, 24, and 48 h. Values for IL-6 -/- + rhIL-6 were significantly different than values for IL-6 -/- at 3, 6, and 16 h. For OATP, values for IL-6 +/+ and IL-6 +/+ + rhIL-6 were significantly different than T0 at 3, 6, 16, 24, and 48 h. Values for IL-6 -/- + rhIL-6 were significantly different than values for T0 at 3, 6, and 16 h. Values for IL-6 +/+ and IL-6 +/+ + rhIL-6 were significantly different than values for IL-6 -/- at 3, 6, 16, 24, and 48 h. Values for IL-6 -/- + rhIL-6 were significantly different than values for IL-6 -/- at 3, 6, and 16 h. Differences within and between groups were analyzed using ANOVA with Bonferroni correction.