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. 2008 Nov 27;57(5):832–845. doi: 10.1111/j.1365-313X.2008.03730.x

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© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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In situ localization of putative ABA-related transcript or transcripts associated with the establishment of the floral meristem in the soybean shoot apices.

(a, b) Signals related to the expression of an ABA-responsive element binding factor (ABRE; GmaAffx.32011.1.S1_at) were detected in the region surrounding the vascular system of the shoot apical meristem (SAM) after 4 short days (SDs) (b), but not in that of a vegetative meristem (0 SD; a).

(c, d) Expression of the SHOOT MERISTEMLESS (STM; Gma.15157.1.A1_at) transcript, a meristem regulatory gene, was found in the SAM and in the axillary meristem during the vegetative stage. The transcript is later expressed strongly in the floral meristem, suggesting an important role in vegetative as well as floral meristem development.

(e, f) Expression of TREHALOSE-PHOSPHATE SYNTHASE (TPS; GmaAffx.21003.1.S1_at) was detected in the axillary meristem during the vegetative phase of growth (e). The signal was later detected in the floral meristem (f). All are longitudinal sections of paraffin-embedded shoot apices probed with the antisense strand of digoxigenin-labelled RNA.