Fig. 1. Far-UV CD spectra of WT (solid line), V53C-pyrene (dashed line), and F229C-pyrene (dotted line) apoA-I in the lipid-free state.
The inset shows the increase in α-helix content of the apoA-I variants bound to egg PC SUV as a function of the weight ratio of PC to apoA-I. ▲, WT apoA-I; □, apoA-I V53C-pyrene; • apoA-I F229C-pyrene. Protein concentration was 50 μg/ml.