Figure 1.

Comparison of colony morphologies for wild-type C. parasitica strain EP155 and corresponding mutants, heterokaryon, and complemented strains. When grown on PDA, mutant bdmA had a phenotype indistinguishable from that previously reported (18) for Gβ gene cpgb-1 disruptant mutants (Δcpgb-1). Heterokaryons formed by anastomosis between bdmA and Δcpgb-1 had an overall phenotype very similar to that of wild-type strain EP155. bdmA transformants complemented with a 6.5-kilobase (kb) KpnI fragment containing the gene bdm-1 were indistinguishable from wild-type strain EP155. Disruption of bdm-1 in strain EP155 (Δbdm-1) resulted in a phenotype indistinguishable from bdmA and the Gβ null mutant Δcpgb-1.