Figure 8.

Measurement of CII-specific Ab by ELISA after immunization. Sera from G-ARKO, B-ARKO, and Wt mice. Mice were immunized with collagen II, and after 21 d the sera were collected and analyzed for the presence of total anti-body of (A) bCII (bCII-specific), (B) mCII (mCII-specific), and (C–F) analysis of isotype expression in the mCII-specific Ab response. mCII-specific isotypes were quantitated using the sera described in panel A and a solid phase ELISA in which the detection Abs were specific for the isotypes listed. The quantity of Ab detected is expressed as relative OD₄₅₀ based on a CII-specific reference serum. Data are presented as mean ± sd. *, P < 0.05.