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PDGF-induced Akt and ERK activation in Control and KD cells. Serum-starved cells were treated with PDGF (0–5 ng/ml) for 10 min before detergent extraction. Equal amounts of protein were resolved by SDS-PAGE and sequentially blotted with anti-IRS-1, anti-PDGFR, anti-P-Akt, and anti-P-ERK.
