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Subcellular distributions of GHR and downstream signaling elements in Control and KD cells. PNS, PM, NLR, and LR fractions were isolated from serum-starved cells, as in Materials and Methods. Equal amounts of protein (5 μg) from each fraction were resolved by SDS-PAGE and sequentially immunoblotted with anti-IRS-1, anti-GHR, anti-ERK and anti-IGF-1R β-chain. The experiments shown are representative of three such experiments. C, Control.
