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Subcelluar distribution of the P-STAT5 and P-ERK in Control and KD cells. Serum-starved cells were treated with GH (500 ng/ml) or vehicle for 10 min at 37 C before harvesting and subcellular fractionation, as in Materials and Methods. Equal amounts of protein (5 μg) from the PNS, PM, NLR, and LR fractions were resolved by SDS-PAGE and sequentially immunoblotted with anti-P-STAT5 and anti-P-ERK. The experiments shown are representative of three such experiments. C, Control.
