Figure 2.

EMSA results showing protein binding activity of nuclear protein to oligonucleotide probes representing the consensus DRE, AP-1, and C/EBP sequences. A, C, and E, MCF10A cells were treated with 10 nm TCDD for different time periods as indicated, and the increase of binding activities to DRE, AP-1, and C/EBP probes was shown. B, MCF10A cells were treated with 10 nm TCDD for 1 h and antibodies against AhR and ARNT were added to show the shift bands. D, MCF10A cells were treated with 10 nm TCDD for 6 h, and antibody against c-Jun was added to show the shift bands. F, MCF10A cells were treated with 10 nm TCDD for 24 h, and antibody against C/EBP-β was added to show the shift bands. Ab, Antibody.