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. 2009 Jan 15;23(4):549–558. doi: 10.1210/me.2008-0317

Figure 5.

Figure 5

Effect of protein kinase inhibitors and siRNA transfection against C/EBP-β and c-Jun on the action of TCDD. A, MCF10A cells were preincubated with 10 μm MNF, 2 μm H89, or 2 μm PP2, respectively, for 30 min and then treated with 10 nm TCDD for 24 h. The mRNA induction levels of selected genes were measured. The results are expressed as fold of induction after TCDD treatment, and the statistically significant differences between the TCDD-only group and other groups are indicated by * (P < 0.05) or ** (P < 0.01). B and C, MCF10A cells were preincubated with 10 μm MNF, 2 μm H89, or 2 μm PP2, respectively, for 30 min and then treated with 10 nm TCDD for 6 h (for AP-1 probe) or 24 h (for C/EBP probe), and the binding activities to those probes were shown. D and E, MCF10A cells were first transfected with 100 nm of scrambled siRNA (as control), si-C/EBP-β, or si-c-Jun, respectively, for 48 h. C/EBP-β and c-Jun mRNA expression levels were measured after siRNA transfection. Cells were then treated with 10 nm TCDD for 24 h. The mRNA induction levels of selected genes were measured. The results are expressed as fold of induction after TCDD treatment, and the statistically significant differences between the scrambled siRNA + TCDD group and other groups are indicated by * (P < 0.05) or ** (P < 0.01).