Figure 6.

PKA activation by TCDD in comparison with FSK. A, MCF10A cell were treated with 10 μm FSK or 10 nm TCDD for different time periods as indicated. PKA activities were measured as described. B, Cells were preincubated with 2 μm H89 for 30 min followed by 10 μm FSK for 1 h. C, Cells were preincubated with 10 μm MNF, 10 μm EGTA/AM, 20 μm MAFP, 2 μm H89, or 2 μm PP2 for 30 min followed by 10 nm TCDD for 6 h. The mRNA induction levels of selected genes were measured. The statistically significant differences between the control and treatment are indicated by * (P < 0.05) or ** (P < 0.01).