Figure 1.

Expression of Star mRNA with short and long 3′-UTR after stimulation of mouse MA-10 cells and primary bovine adrenocortical cells. A, Mouse and bovine AURE regions with TIS11b recognition sequences highlighted. B, MA-10 cells were treated with Br-cAMP (0.4 mm) for the indicated times. Cells were harvested and total RNA subjected to Northern blot analysis. Expression of 28S RNA was monitored and similar in each sample. C, BAC were treated for the indicated times with and without ACTH (10 nm). Northern blots for Star mRNA are compared along with quantification of total mRNA, using 18S rRNA as a loading control.