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. 2009 Jan 29;23(4):497–509. doi: 10.1210/me.2008-0296

Figure 5.

Figure 5

Degradation of directly transfected 3.5-kb Star mRNA is selectively enhanced by Br-cAMP stimulation but is independent of AURE mutations. A, Diagram showing experimental design. Rat Star mRNA with different 3′-UTR was transcribed from Star cDNA, which each had 90-base poly-A tails. The mRNA was transfected into MA-10 cells, and the time course for entry into MA-10 cells was determined by RT-PCR. Steady-state expression was attained after 12 h. Cells were treated with or without Br-cAMP at the time of mRNA transfection and also after a wash removal of the liposome/mRNA mix. Cells were lysed at the indicated times. B, First-order decay kinetics (log Star mRNA/total RNA vs. time) are shown for representative experiments, with or without Br-cAMP. C, Half-lives for each mRNA species calculated by linear regression fit of the time points on semi-log plots. Included is a second experiment in which 3.5-kb mRNA is mutated at two AURE sites (StAR3.5k12m). §, P < 0.05 for basal half-life compared with Star no-UTR control; *, P < 0.05 for effects of Br-cAMP.