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Functional validation of V2R Rluc8-tagged BRET fusion protein. cAMP was measured after addition of AVP (treated) or vehicle (untreated) in COS-7 cells transfected with untagged V2R, V2R tagged with Rluc8 (V2R/Rluc8), or empty vector (control). Data shown are mean ± sem of three independent experiments.
