Table 1.

Relative luminescence intensities of the three V2R mutants investigated compared with the luminescence intensity of V2R-wild type transfected with the corresponding β-arrestin 2 construct (β-arrestin 2/Venus or β-arrestin 2/GFP10)

Transfected constructs	Luminescence relative to V2R-wild type/Rluc8 (%)
β-Arrestin 2/Venus and V2R-R137C/Rluc8	84.9 ± 3.1
β-Arrestin 2/Venus and V2R-R137L/Rluc8	101.0 ± 4.1
β-Arrestin 2/Venus and V2R-R137H/Rluc8	77.7 ± 3.4
β-Arrestin 2/GFP10 and V2R-R137C/Rluc8	95.7 ± 1.1
β-Arrestin 2/GFP10 and V2R-R137L/Rluc8	99.8 ± 3.5
β-Arrestin 2/GFP10 and V2R-R137H/Rluc8	85.3 ± 4.7

Luminescence intensities were detected as part of the BRET kinetic assay. Data shown are mean ± sem of four independent experiments.