Table 2.

Relative luminescence intensities of the V2R-wild type and three V2R mutants investigated compared with the luminescence intensity of the corresponding Rluc8-tagged nontruncated receptors cotransfected with β-arrestin 2/Venus

Transfected constructs	Luminescence relative to Rluc8-tagged nontruncated receptors (%)
β-Arrestin 2/Venus and V2R-trunc/Rluc8	108.2 ± 2.0
β-Arrestin 2/Venus and V2R-R137C-trunc/Rluc8	86.5 ± 3.6
β-Arrestin 2/Venus and V2R-R137L-trunc/Rluc8	128.6 ± 9.2
β-Arrestin 2/Venus and V2R-R137H-trunc/Rluc8	111.2 ± 16.2

Luminescence intensities were detected as part of the BRET kinetic assay. Data shown are mean ± sem of four independent experiments.