Figure 2.

Real-time RT-PCR validation of DASL results. LNCaP cells were seeded in medium supplemented with charcoal-stripped serum. Medium was changed 2 d later, and cells were treated with 0.1, 1, or 10 nm of the synthetic androgen R1881 (black bars) or ethanol vehicle (gray bars) for 48 h. RNA was isolated and converted into cDNA. Real-time RT-PCR was performed with the primer pairs listed in supplemental Table 3. Coregulator mRNA levels were normalized with the values obtained from glyceraldehyde-3-phosphate dehydrogenase . Values are expressed as relative expression levels, taking the value obtained from one of the untreated samples as 1. Columns indicate means of values obtained from three independent biological replicates; bars, sem values.