Figure 4.

Time course of the effects of androgens on coregulator protein expression. LNCaP cells were seeded in charcoal-stripped serum medium. Medium was changed 2 d later, and cells were treated with 1 nm of R1881 (+) or vehicle (−) for the indicated periods of time. Total protein extracts were prepared, and equal amounts of protein were analyzed by Western blotting using antibodies directed against PB1, CBP, SRC1, SRC2, SRC3, or XRCC5. To evaluate potential loading differences, blots were stripped and reprobed with an antibody against β-actin (b-act).