Figure 6.

Western blot analysis of differential expression of coregulators in isogenic LNCaP cells. LNCaP and LNCaP-Rf cells were seeded in their regular medium. Total protein extracts were prepared 3 d later, and equal amounts of protein were analyzed by Western blotting using antibodies directed against PB1, CBP, SRC1, SRC2, SRC3, or XRCC5. To evaluate potential loading differences, blots were stripped and reprobed with an antibody against β-actin (b-act).