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Loss of coregulator expression does not markedly affect AR protein expression. LNCaP cells were transfected with specific siRNAs targeting coregulator expression or control (c) siRNAs. Medium was changed 12–16 h after transfection. Proteins were isolated 96 h later, and Western blotting was performed using an antibody directed against the AR. To assess potential intersample loading differences, blots were stripped and reprobed with antibodies recognizing β-actin (b-act).
