Figure 9.

Role of coregulator expression in androgen-dependent cell proliferation. LNCaP cells were transfected with siRNAs targeting the expression of PIAS1, MAGEA11 (MAGE) or NCOA7, or control siRNAs (c). Cells were treated 16 h after transfection with 0.1, 1 or 10 nm R1881 (black bars) or vehicle bars (gray bars). Cell proliferation was assessed 24, 48, 72, and 96 h later by means of an MTS assay reading absorbance at 490 nm. Columns represent mean values from five individual measurements; bars, sem values.