Table 1.
Effect of androgen treatment on DASL expression profile: androgen target genes
| Gene symbol | Fold induction
|
|
|---|---|---|
| 0.1 nm | 1.0 nm | |
| KLK2 | 6.552 | 9.633 |
| MSMB | 4.231 | 3.538 |
| ACPP | 0.649 | 1.100 |
| TMPRSS2 | 1.924 | 3.408 |
| ODC1 | 1.051 | 2.181 |
| AMD1 | 1.100 | 1.179 |
| SMS | 1.166 | 1.198 |
| SAT | 1.056 | 1.251 |
| ACACA | 1.400 | 1.452 |
| ACACB | 2.336 | 0.813 |
| ME1 | 1.317 | 1.699 |
| FASN | 0.880 | 3.797 |
| HMGCS1 | 1.120 | 1.308 |
| HMGCS2 | 1.516 | 1.862 |
| HMGCR | 1.097 | 2.052 |
| DHCR24 | 1.783 | 4.296 |
| FDPS | 1.221 | 1.209 |
| SREBF1 | 0.684 | 1.202 |
| SREBF2 | 1.072 | 1.553 |
| SCAP | 1.094 | 1.294 |
| INSIG1 | 1.310 | 2.351 |
| INSIG2 | 0.721 | 0.604 |
| ACTA2 | 1.327 | 4.317 |
| ACTG2 | 2.180 | 5.963 |
| MYL9 | 0.518 | 3.074 |
| MYLK | 0.275 | 0.294 |
| TPM1 | 0.775 | 1.328 |
| FN1 | 0.753 | 0.221 |
| NKX3-1 | 2.133 | 2.749 |
| CDK8 | 0.612 | 0.414 |
| BCL2 | 1.207 | 0.769 |
| CASP2 | 1.060 | 0.837 |
| PIK3R3 | 0.917 | 0.467 |
| RAB4A | 1.072 | 1.333 |
| LIFR | 1.156 | 3.006 |
| HERC3 | 1.413 | 3.013 |
| VEGFA | 1.025 | 3.006 |
| VEGFC | 2.357 | 0.904 |
| NDRG1 | 1.358 | 5.378 |
| NFKBIA | 0.869 | 1.167 |
| BCHE | 0.763 | 0.306 |
| AR | 0.526 | 0.407 |
The order of the genes listed corresponds to the order in which the genes are listed and grouped in the heatmap shown in supplemental Fig. 1. LNCaP cells were seeded in medium supplemented with charcoal-stripped serum. Medium was changed 3 d later, and cells were treated with 0, 0.1 or 1 nm of R1881. Cells were harvested 48 h later, and RNA was isolated and subjected to DASL analysis. Values represent the average fold change in gene expression after treatment with 0.1 or 1 nm of R1881 from three biological replicates each.