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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: J Immunol. 2009 Mar 15;182(6):3469–3481. doi: 10.4049/jimmunol.0801814

FIGURE 10.

FIGURE 10

The number of transferred Tc17 after influenza A infection. Tc17 effectors from OT-1.CD45.1 were prepared and 2 × 106 of Tc17 were i.v. injected into naive B6.Thy1.1 recipients. One day after Tc17 injection, B6.Thy1.1 mice were i.n. infected with ∼0.3 LD50 of influenza A/PR8oval. Mice were sacrificed at the times indicated following challenge, and cells suspensions were prepared from spleen (□), lung (△), MedLN (▽), ILN (◇), and BAL (○) and restimulated with PMA/ionomycin. After restimulation, cells were stained with Abs to intracellular IL-17 and surface CD8. The analysis was gated on CD45.1+CD45.2-CD90.1-CD90.2+ CD8 T cells to exclude other cell types.