FIGURE 4.

Not4p is not involved in the degradation of nonstop products. W303 or W303not4Δ cells were transformed with the indicated pGPDp-GFP-FLAG-HIS3 (pSA156) or pGPDp-GFP-FLAG-HIS3-NS (pSA157) plasmids (4). A, the cells were grown in SC-Ura, and the samples were analyzed using Western blotting with anti-GFP antibodies. B, the cells were grown in SC-Ura, and the samples were analyzed using Northern blotting with DIG-labeled GFP and SCR probes. WT, wild type.