. 2009 Apr 17;284(16):10353–10360. doi: 10.1074/jbc.M900521200

TABLE 2.

Oligonucleotides used to generate recombinant TodS fragments and site-directed mutants

Plasmids	Sequence (5′-3′)
For expression plasmids of TodS fragments
pHK1	GGGGCTAGCAATTATTATAATATCTGCCTCAAG
	CGCGGATCCTCAGTCGGATTGAAATGGAAG
pRRR	GAGGCTAGCCAGCCTCGGGTGCTAAT
	CACGGATCCTCACCCCTGATCGGAAAGCTC
pHK2	GCATACGGATCCTCACTGTCTGGCAGGGATACT
For site-directed mutagenesis of plasmids pTodS and pMIR66
pAB1 and pAB4	TTGCCAAGGTGAGCGCTGAGTTGCGCACTCC^a
	GGAGTGCGCAACTCAGCGCTCACCTTGGCAA^a
pAB2 and pAB5	ACCTGTTGATTACAGCCCTGATGATGCCT^a
	AGGCATCATCAGGGCTGTAATCAACAGGT^a
pAB3 and pAB6	GCAGCCTATATTGCAGCCGAGATAAACCAACCG^a
	CGGTTGGTTTATCTCGGCTGCAATATAGGCTGC^a

Mismatch nucleotides for amino acid replacement to alanine are underlined.