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. 2009 Apr 17;284(16):10361–10366. doi: 10.1074/jbc.M900956200

FIGURE 1.

FIGURE 1.

Interaction between DBC1 and SUV39H1. a, H1299 cells were transiently transfected with the indicated plasmids for 48 h. Endogenous DBC1 was immunoprecipitated with anti-DBC1 antibody. Coprecipitated SUV39H1 was detected by anti-Myc Western blotting (WB). DBC1, Myc-MDMX, and Myc-SUV39H1 expression levels were confirmed in WCE by anti-DBC1 and anti-Myc antibodies. b, SirT1 was immunoprecipitated with antibody 10E4, whereas SUV and MDMX were immunoprecipitated with anti-Myc antibody. Coprecipitated DBC1 was detected by anti-DBC1 Western blotting. SUV39H1 and MDMX were detected by anti-Myc Western blotting. c, HA-DBC1 was cotransfected into H1299 cells with FLAG-tagged histone methyltransferases and immunoprecipitated with anti-HA antibody. Coprecipitated enzymes were detected by anti-FLAG Western blotting. Expression of HA-DBC1 in the WCE was detected with anti-DBC1 antibody. d, H1299 cells were transiently transfected with the indicated plasmids. SUV39H1 was immunoprecipitated with mouse anti-SUV39H1 antibody. Coprecipitated SirT1 and DBC1 were detected with antibody 10E4 and anti-DBC1 antibody, respectively. SUV39H1 was detected by reprobing with rabbit anti-SUV antibody.