Skip to main content
. 2009 Apr 17;284(16):10361–10366. doi: 10.1074/jbc.M900956200

FIGURE 5.

FIGURE 5.

DBC1 inhibits SUV39H1 in vivo. a, H1299 cells were transfected with DBC1 siRNA or control siRNA. Histones were extracted, and the H3K9me3 level was detected with anti-H3K9me3 antibody and reprobing with anti-H3 antibody. DBC1 in the WCE was detected with anti-DBC1 antibody. b, 293T cells were transiently transfected with the indicated plasmids for 48 h. Histone was extracted, and H3K9me3 was detected with anti-H3K9me3 antibody. DBC1 in the WCE was detected with anti-DBC1 antibody. SUV39H1 in the WCE was detected with anti-Myc antibody. c, H1299 cells were transfected with the indicated plasmids for 48 h. Relative luciferase activity was determined and normalized by cotransfected lacZ control. d, H1299 cells were transiently transfected with the indicated plasmids for 48 h. Acetylation of p53 Lys382 was detected with anti-Ac-K382 antibody and reprobed with p53 antibody DO1. e, H1299 was transfected with the indicated plasmids for 48 h. Cell lysate was analyzed for histone deacetylase (HDAC) activity using a 3H-labeled acetylated H3 peptide as substrate. f, a model of DBC1 regulation of the SUV39H1-SirT1 complex is shown. Error bars represent S.D. from three experiments.