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. 2009 Apr 17;284(16):10367–10375. doi: 10.1074/jbc.M807822200

FIGURE 1.

FIGURE 1.

Generation of IRAK2-deficient mice. A, structure of the mouse IRAK2 gene and the targeted IRAK2 gene. B, PCR analysis of IRAK2 genomic DNA in wild type and knock-out mice. KO, knock-out. C, BM-derived macrophages (BMM) from wild type and IRAK2-deficient mice were untreated or treated with R848 (1 μg/ml). Total RNA (10 μg) was analyzed by the Northern method with a probe specific for IRAK2. IRAK2 mRNA was induced by R848 in BM-derived macrophages from wild type but not IRAK2-deficient mice. D, BMM from WT and KO mice were untreated or treated with LPS (1 μg/ml) or R848 (1 μg/ml). Cell lysates were analyzed by Western method with an antibody against IRAK2. Stimulation by either LPS or R848 led to modification of IRAK2 in macrophages from wild type mice. IRAK2 protein was absent in IRAK2-deficient macrophages.