FIGURE 4.

Impaired LPS-mediated mRNA stabilization in IRAK2-deficient macrophages. A, WT and IRAK2-deficient (KO) BM-derived macrophages were pretreated with LPS (1 μg/ml) for 90 min and then treated with actinomycin D (Act D, 5 μg/ml) and LPS (1 μg/ml) for indicated times. KC, IL-6, and TNFα and mRNAs were analyzed by quantitative reverse transcription-PCR, normalized by β-actin, and plotted. The results shown are the mean ± S.D. of three independent experiments. *, p < 0.05. B, WT and IRAK2-deficient (KO) BM-derived macrophages were either untreated (NT) or pretreated with LPS (1 μg/ml) for 90 min and then treated with actinomycin D (5 μg/ml) and LPS (1 μg/ml) for the indicated times. Total RNAs (10 μg) were analyzed by the Northern method.