FIGURE 5.

Impaired LPS-mediated protein translation in IRAK2-deficient macrophages. A, translation-active and -inactive mRNAs from LPS-treated BM-derived macrophages were isolated by sucrose gradient fraction. B, BM-derived macrophages from wild type and IRAK2-deficient mice were treated with LPS (1 μg/ml) for 1.5 h. Cytokine, chemokine, and glyceraldehyde-3-phosphate dehydrogenase mRNAs from unfractionated cell lysates, translation-active pools, and translation-inactive pools were analyzed by quantitative reverse transcription-PCR and normalized to β-actin. Similar results were obtained in three independent experiments. One representative is shown. C, the ratios of TNFα, IL-6, and KC mRNA from translation-active and -inactive pools are shown. The results shown are the means ± S.D. of three independent experiments. *, p < 0.05.