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. 2009 Apr 17;284(16):10433–10445. doi: 10.1074/jbc.M809801200

FIGURE 7.

FIGURE 7.

The ratio of SPFH1 to SPFH2 in the SPFH1/2 complex is flexible. A, lysates from Rat-1 cells expressing the indicated shRNAs were prepared in 1% Triton X-100-containing lysis buffer, were subjected to BN-PAGE, and were probed for SPFH1 or SPFH2. B, anti-SPFH1 or anti-SPFH2 IPs from Rat-1 cells expressing the indicated shRNAs were Coomassie Blue stained, and bands corresponding to SPFH1 and SPFH2 are marked. C, HeLa cells were transfected with varying amounts of SPFH1-FLAG and SPFH2-HA cDNA, anti-HA IPs were Coomassie Blue stained, and bands corresponding to SPFH1-FLAG and SPFH2-HA are marked. IP, immunoprecipitate; IB, immunoblot.