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. 2009 Apr 17;284(16):10446–10452. doi: 10.1074/jbc.M900096200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Schematics of putative SIK-mediated phosphorylation sites on mouse nSREBP-1c sequences. A, putative phosphorylation sites by SIK kinases on mouse SREBP-1c nuclear form are shown. Each mutant construct is designed as an alanine substitution for the serine residue indicated. B, in vitro kinase assay was performed with purified GST-fused nSREBP-1c proteins and immunoprecipitated FLAG-tagged SIK1. Effects of specific alanine substitution mutations on SREBP-1c for SIK-induced phosphorylation were shown. C, comparison of amino acid sequences for nSREBP-1c within the SIK target region (serine 265/266 and serine 329) among humans, mice, and rats. 3A, S265A/S266A/S329A.