TABLE 1.
Microarray analysis showing hepatic expression patterns of metabolic genes by SIK1 overexpression or knockdown
Microarray analysis was performed using RNAs from mouse liver infected with Ad-SIK1 or Ad-GFP and with Ad-SIK1 RNAi or Ad-US control RNAi adenoviruses. Genes involved in each metabolic pathway are listed. Lipogenic genes are as follows: Srebf1, sterol regulatory element-binding transcription factor 1; Acaca, acetyl-CoA carboxylase α; Fasn, fatty acid synthase; Thrsp, thyroid hormone-responsive SPOT14 homolog; Gpd1, glycerol-3-phosphate dehydrogenase 1. Gluconeogenic genes are as follows: G6pc, glucose-6-phosphatase, catalytic subunit; Pcx, pyruvate carboxylase. Genes involved in the cholesterol synthesis or uptake are as follows: Pmvk, phosphomevalonate kinase; Hmgcs1, 3-hydroxy-3-methylglutaryl-coenzyme A synthase 1; Fdps, farnesyl diphosphate synthetase; Ldlr, low density lipoprotein receptor; Acy1, aminoacylase 1; Ggps1, geranylgeranyl diphosphate synthase 1.
|
Gene
|
-Fold induction
|
|
|---|---|---|
| SIK1 versus GFP | SIKI RNAi versus US | |
| Srebf1 | 0.571 | 1.347 |
| Acaca | 0.991 | 1.764 |
| Fasn | 0.652 | 1.416 |
| Thrsp | 0.680 | 7.360 |
| Gpd1 | 0.622 | 1.502 |
| G6pc | 0.819 | 1.399 |
| Pcx | 0.853 | 1.257 |
| Pmvk | 0.952 | 0.852 |
| Hmgcs1 | 1.337 | 0.683 |
| Fdps | 0.741 | 0.832 |
| Ldlr | 1.125 | 1.080 |
| Acy1 | 0.959 | 1.051 |
| Ggps1 | 1.234 | 1.15 |