FIGURE 1.

Response of adipocyte apoE and LXR expression to rosiglitazone. A, 3T3-L1 adipocytes were treated with the indicated dose of rosiglitazone (Rosi) for 48 h. B, 3T3-L1 adipocytes were treated with 10 μm rosiglitazone or vehicle for the time indicated. The -fold change at each time point is relative to cells treated for the same time with vehicle alone. At the end of the incubation, total RNA was isolated and apoE mRNA was measured as described under “Experimental Procedures.” Values shown are mean ± S.D. C, 3T3-L1 cells were treated with 10 μm rosiglitazone for 48 h. 50 μg of total cell protein was used for immunoblot using an antibody to LXR. D, 30 μg of nuclear protein was used for immunoblot.