FIGURE 2.

Activated, intracellular, Gα_s-GFP colocalizes with microtubules. PC12 cells expressing Gα_s-GFP were treated with vehicle (Control), cholera toxin (CTX), or forskolin and fixed with cold methanol after extraction with saponin. Microtubules were visualized with a α-tubulin antibody and a rhodamine-conjugated secondary antibody. Cells were examined with deconvolution microcopy. Yellow shows the overlay of Gα_s-GFP along microtubules. Tubulin (red) and Gα_s-GFP (green) are also shown in the image. Bar, 10 μm.