FIGURE 6.
Constitutively active GαsQ227L reduces microtubule-stabilizing modifications in PKA-deficient 123.7 PC12 cells. A, post-translational modification of tubulin in 123.7 cells. The cells were infected with adenoviruses and lysed, and proteins were analyzed by immunoblotting with anti-acetylated tubulin (Ace-tubulin), anti-detyrosinated tubulin (Glu-tubulin), anti-tyrosinated tubulin (Tyr-tubulin), or anti-α-tubulin antibodies. B, quantitative analysis. Levels of post-translationally modified tubulin were quantified using scanning densitometry of blots, and data were normalized to percentage of control. All values represent the mean ± S.E., n = 3. *, p < 0.05 versus control.