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. 2009 Apr 17;284(16):10473–10479. doi: 10.1074/jbc.M809106200

FIGURE 4.

FIGURE 4.

S77 affects the formation, but not stability, of the alternative pathway C3 convertase. A, S77 inhibits binding of factor B to C3b. C3b was coated on microtiter plates. S77 or control Fab was added, followed by addition of factor B. Binding of factor B to C3b was determined with anti-factor B antibody and secondary antibodies conjugated to HRPO. B, S77 Fab inhibits formation of C3 convertase. C3b was coated on microtiter plates. S77 or control Fab was added, followed by addition of fB and factor D. Binding of fBb to C3b was determined with anti-fB antibody and secondary antibody conjugated to HRPO. C, S77 does not decay the C3 convertase. Microtiter plates were coated with C3b, incubated with factor B and factor D followed by the addition of S77, sCR1 or control Fab. Factor Bb was detected with goat anti-human factor B and donkey anti-goat antibody conjugated to HRPO. Absorbance of the reaction product was read at 450 nm. Data are expressed as mean ± S.D. of four repeats.